PCK1 antibody

Catalog No. ABIN2460581

The Rabbit Polyclonal anti-PCK1 antibody is suitable to detect PCK1 in samples from Human, Mouse, Rat and Dog. It has been validated for WB, ELISA and IHC.

Quick Overview for PCK1 antibody (ABIN2460581)

Target: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PCK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-PCK1 Antibody

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PCK1.

Application Details

Application Notes: PCK1 antibody can be used for detection of PCK1 by ELISA at 1:1562500. PCK1 antibody can be used for detection of PCK1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store PCK1 antibody at -20 °C.

Target Details for PCK1

Target: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Alternative Name: PCK1

Background: PCK1 is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of PCK1 can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet.

Molecular Weight: 69 kDa

Gene ID: 5105

NCBI Accession: NP_002582

UniProt: P35558

Pathways: Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis