HMGB1 antibody

Catalog No. ABIN2461647

The Rabbit Polyclonal anti-HMGB1 antibody (ABIN2461647) specifically detects HMGB1 in WB and ELISA. The antibody is reactive with Human, Mouse, Rat and Dog samples.

Quick Overview for HMGB1 antibody (ABIN2461647)

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HMGB1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-HMGB1 Antibody

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HMGB1.

Application Details

Application Notes: HMGB1 antibody can be used for detection of HMGB1 by ELISA at 1:62500. HMGB1 antibody can be used for detection of HMGB1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store HMGB1 antibody at -20 °C.

Target Details for HMGB1

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Alternative Name: HMGB1

Background: Extracellular HMGB1 is an activator of human tumor cell migration operating in concert with EGF. HMGB1 encodes a protein that is potentially involved in the regulation of lipogenic and cholesterogenic gene transcription.

Molecular Weight: 25 kDa

Gene ID: 3146

NCBI Accession: NP_002119

UniProt: P09429

Pathways: p53 Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome