TARDBP antibody

Catalog No. ABIN2461819

This Rabbit Polyclonal antibody specifically detects TARDBP in WB, IHC and ELISA. It exhibits reactivity toward Human, Mouse, Rat and Dog.

Quick Overview for TARDBP antibody (ABIN2461819)

Target: TARDBP (TAR DNA Binding Protein (TARDBP))

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TARDBP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-TARDBP Antibody

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TARDBP.

Application Details

Application Notes: TARDBP antibody can be used for detection of TARDBP by ELISA at 1:1562500. TARDBP antibody can be used for detection of TARDBP by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store TARDBP antibody at -20 °C.

Target Details for TARDBP

Target: TARDBP (TAR DNA Binding Protein (TARDBP))

Alternative Name: TARDBP

Background: HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. TARDBP is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription.HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20.

Molecular Weight: 45 kDa

Gene ID: 23435

NCBI Accession: NP_031401

UniProt: Q13148

Pathways: Positive Regulation of Peptide Hormone Secretion