APOL1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
APOL1 antibody was raised against a amino acid synthetic peptide near the of APOL1 (aa 297-309).
APOL1
Reactivity: Human
WB, IHC (p)
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:16000. Western Blot: Approximately 42+45 kDa bands observed in human placenta lysates (calculated MW of 45.9 kDa and 42.2 kDa according to NP_663318.1 and NP_001130013.1 resp.). Recommended concentration: 0.1-0.3 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.