Western Blotting (WB), Immunohistochemistry (IHC), ELISA
Purification
RBP1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
RBP1 antibody was raised against a 14 amino acid synthetic peptide near the C-Terminus of RBP1.
ELISA: Antibody detection limit dilution 1:16000. Western Blot: Approximately 16 kDa band observed in Murine NIH 3T3 lysates (calculated MW of 15.9 kDa according to NP_002890.1). Recommended concentration: 0.1-0.3 μg/mL. Immunohistochemistry: In paraffin embedded human pancreas shows vesicular staining in the acinar cytoplasm but oriented towards the interlobular space. Recommended concentration, 5-10 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.