ODZ3 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
ODZ3 antibody was raised against a 13 amino acid synthetic peptide near the internal region of ODZ3.
ODZ3
Reactivity: Human, Mouse, Rat
IF (p)
Host: Rabbit
Polyclonal
Alexa Fluor 750
Application Notes
ELISA: Antibody detection limit dilution 1:16000. Western Blot: Preliminary experiments in human brain lysates and in lysates of cell line NIH 3T3 gave no specific signal but low background (at antibody concentration up to 1 μg/mL). Immunohistochemistry: In paraffin embedded human cerebellum shows nuclear staining of the granular cell layer. Recommended concentration, 1.5-3 μg/mL. Immunofluorescence: Nuclear staining (green) in HeLa cells.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.