GFP antibody (full length)

Catalog No. ABIN2467764

This anti-GFP antibody is a Rabbit Polyclonal antibody detecting GFP in WB, ELISA and IHC. Suitable for Aequorea victoria.

Quick Overview for GFP antibody (full length) (ABIN2467764)

Target: GFP (Green Fluorescent Protein (GFP))

Reactivity: Aequorea victoria

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GFP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-GFP Antibody

Binding Specificity: full length

Purification: Anti-GFP Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria). No reaction was observed against Human, Mouse or Rat serum proteins.

Immunogen: Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.

Application Details

Application Notes: Polyclonal anti-GFP is designed to detect GFP and its variants. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-GFP to detect GFP or GFP containing proteins on western blots. Recommended dilutions are as follows, ELISA 1:15,000 - 1:60,000, Western Blot 1:500 - 1:5,000, Immunohistochemistry 1:200 - 1:3,000. (Optimal dilutions should be determined by user).

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, 0.01 % (w/v) Sodium Azide, pH 7.2

Handling Advice: Avoid freeze thaw cycles

Storage: -20 °C

Storage Comment: Store vial at -20 C or below prior to opening. Dilute only prior to immediate use. Forextended storage, aliquot contents and freeze at -20 C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening product.

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: GFP

Gene ID: 7011691

NCBI Accession: YP_002302326

UniProt: P42212