RAD52 antibody (AA 122-135)

Catalog No. ABIN2470268

The Rabbit Polyclonal anti-RAD52 antibody is suitable to detect RAD52 in samples from Human. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for RAD52 antibody (AA 122-135) (ABIN2470268)

Target: RAD52 (RAD52 Homolog (S. Cerevisiae) (RAD52))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAD52 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-RAD52 Antibody

Binding Specificity: AA 122-135

Purification: Immunoaffinity Chromatography

Immunogen: RAD52 antibody was raised against amino acids 122 - 135 of RAD52 (Human).

Application Details

Application Notes: RAD52 antibody can be used in Western Blot at 1:1000, immunohistochemistry starting at 1:25 - 1:100, and immunoprecipitation starting at 1:50.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: As with all antibodies avoid freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Store RAD52 antibody at -20 °C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20 °C or below.

Target Details for RAD52

Target: RAD52 (RAD52 Homolog (S. Cerevisiae) (RAD52))

Alternative Name: RAD52

Background: The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair. Four alternatively spliced transcript variants encoding different isoforms have been reported for this gene. The alpha variant encodes the longest isoform that contains an identical N-terminus, but a distinct C-terminus, as compared to other isoforms (beta, delta, and gamma).

Gene ID: 5893

UniProt: P43351

Pathways: DNA Damage Repair