CDK2 antibody

Catalog No. ABIN2470565

The Mouse Monoclonal anti-CDK2 antibody is suitable to detect CDK2 in samples from Human, Mouse and Xenopus laevis. It has been validated for WB, IHC, ELISA and IP.

Quick Overview for CDK2 antibody (ABIN2470565)

Target: CDK2 (Cyclin-Dependent Kinase 2 (CDK2))

Reactivity: Human, Mouse, Xenopus laevis

Host: Mouse

Clonality: Monoclonal

Conjugate: This CDK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Product Details anti-CDK2 Antibody

Specificity: Recognizes cdk2, Mr 33kD. Does not cross-react with other cdks.

Purification: Protein G Column

Immunogen: CDK2 monoclonal antibody was raised against recombinant CDK2 (Human).

Isotype: IgG

Application Details

Application Notes: CDK2 antibody can be used in immunohistochemistry starting at 10 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05 % sodium azide, before the addition of glycerol to 30 %

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: As with all antibodies avoid freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store CDK2 antibody at 4 °C or -20 °C.

Target Details for CDK2

Target: CDK2 (Cyclin-Dependent Kinase 2 (CDK2))

Alternative Name: CDK2

Background: Cyclin-dependent kinase 2 (CDK2), a CDC2/CDK-type protein kinase, regulates cell-cycle transition from G1 to S phase. CDK2 activation requires formation of a heterodimer with cyclin A, D, or E and phosphorylation by CAK. CDK2 inhibition results from association with p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Two alternatively spliced variants and multiple transcription initiation sites have been reported. Inhibition of CDK2 has been suggested to help prevent chemotherapy-induced alopecia in cancer patients.

Gene ID: 1017

UniProt: P24941

Pathways: PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA