Solute Carrier Family 17 (Vesicular Glutamate Transporter), Member 6 (SLC17A6) (AA 501-582) antibody (Biotin)

Catalog No. ABIN2483743

The Mouse Monoclonal anti-Solute Carrier Family 17 (Vesicular Glutamate Transporter), Member 6 antibody is suitable to detect Solute Carrier Family 17 (Vesicular Glutamate Transporter), Member 6 in samples from Rat. It has been validated for WB, IHC, IF and ICC.

Quick Overview for Solute Carrier Family 17 (Vesicular Glutamate Transporter), Member 6 (SLC17A6) (AA 501-582) antibody (Biotin) (ABIN2483743)

Target: Solute Carrier Family 17 (Vesicular Glutamate Transporter), Member 6 (SLC17A6)

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: S29-29

Product Details

Binding Specificity: AA 501-582

Specificity: Detects ~60 kDa.

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein G Purified

Immunogen: Fusion protein amino acids 501-582 (cytoplasmic C-terminus) of rat VGlut2

Isotype: IgG1

Application Details

Application Notes:

• WB (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN2483743 was sufficient for detection of VGLut2 in 20 μg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details

Target: Solute Carrier Family 17 (Vesicular Glutamate Transporter), Member 6 (SLC17A6)

Alternative Name: VGLUT2

Background: The ATP-dependent, chloride-sensitive vesicular glutamate transporters (VGLUT) include BNPI (VGLUT1), VGLUT2 (DNPI) and VGLUT3. The brain expresses BNPI (brain specific Na+-dependent inorganic phosphate (Pi) cotransporter) and VGLUT2 in a complementary fashion. The telencephalic regions express BNPI, whereas the lower brainstem and diencephalic regions express VLGUT2. Rat pinealocytes express both BNPI and VGLUT2. The striatum, hippocampus, cerebral cortex and raphe nuclei express VGLUT3 in a small number of neurons. Pancreatic α and β cells express BNPI and VGLUT2 in response to glucose concentrations. Human VGLUT3 shares a 72 % sequence homology with VLGUT2 and BNPI.

Gene ID: 84487

NCBI Accession: NP_445879

UniProt: Q9JI12