Solute Carrier Family 17 (Vesicular Glutamate Transporter), Member 6 (SLC17A6) (AA 501-582) antibody (FITC)

Details for Product No. ABIN2483744
Antigen
Epitope
AA 501-582
25
10
7
3
3
3
2
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
75
67
64
2
1
Host
Mouse
56
31
8
Clonality (Clone)
Monoclonal ()
Conjugate
FITC
5
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
76
54
26
23
23
13
5
5
2
2
2
1
Options
Immunogen Fusion protein amino acids 501-582 (cytoplasmic C-terminus) of rat VGlut2
Clone S29-29
Isotype IgG1
Specificity Detects ~60 kDa.
Purification Protein G Purified
Antigen
Alternative Name VGLUT2 (SLC17A6 Antibody Abstract)
Background The ATP-dependent, chloride-sensitive vesicular glutamate transporters (VGLUT) include BNPI (VGLUT1), VGLUT2 (DNPI) and VGLUT3. The brain expresses BNPI (brain specific Na+-dependent inorganic phosphate (Pi) cotransporter) and VGLUT2 in a complementary fashion. The telencephalic regions express BNPI, whereas the lower brainstem and diencephalic regions express VLGUT2. Rat pinealocytes express both BNPI and VGLUT2. The striatum, hippocampus, cerebral cortex and raphe nuclei express VGLUT3 in a small number of neurons. Pancreatic α and β cells express BNPI and VGLUT2 in response to glucose concentrations. Human VGLUT3 shares a 72 % sequence homology with VLGUT2 and BNPI.
Gene ID 84487
NCBI Accession NP_445879
UniProt Q9JI12
Application Notes
  • WB (1:1000)
  • ICC/IF (1:100)
  • optimal dilutions for assays should be determined by the user.
Comment

1 μg/ml of ABIN2483744 was sufficient for detection of VGLut2 in 20 μg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
Image no. 1 for anti-Solute Carrier Family 17 (Vesicular Glutamate Transporter), Member 6 (SLC17A6) (AA 501-582) antibody (FITC) (ABIN2483744) Western Blot analysis of Rat brain membrane lysate showing detection of VGLUT2 protei...
Image no. 2 for anti-Solute Carrier Family 17 (Vesicular Glutamate Transporter), Member 6 (SLC17A6) (AA 501-582) antibody (FITC) (ABIN2483744) Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VGLUT2 Monoclonal An...