NMDAR2A antibody (AA 75-325) (HRP)

Catalog No. ABIN2483898

This Mouse Monoclonal antibody specifically detects NMDAR2A in WB, IHC, IF and ICC. It exhibits reactivity toward Rat.

Quick Overview for NMDAR2A antibody (AA 75-325) (HRP) (ABIN2483898)

Target: NMDAR2A (GRIN2A) (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2a (GRIN2A))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This NMDAR2A antibody is conjugated to HRP

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: S327A-38

Product Details anti-NMDAR2A Antibody (HRP)

Binding Specificity: AA 75-325

Specificity: Detects ~170 kDa. Does not react with NR2B.

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein G Purified

Immunogen: Fusion protein amino acids 75-325 (extracellular N-terminus) of rat GluN2A/NR2A

Isotype: IgG2b

Application Details

Application Notes:

• WB (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN2483898 was sufficient for detection of GluN2A/NR2A in 20 μg of COS cells transiently transfected with GFP-tagged NR2A lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for NMDAR2A

Target: NMDAR2A (GRIN2A) (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2a (GRIN2A))

Alternative Name: NR2A

Background: N-methyl-D-aspartate (NMDA) receptors are a class of ionotropic glutamate-gated ion channels. These receptors have been shown to be involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of the key receptor subunit NMDAR1 (GRIN1) and 1 or more of the 4 NMDAR2 subunits: NMDAR2A (GRIN2A), NMDAR2B (GRIN2B), NMDAR2C (GRIN2C) and NMDAR2D (GRIN2D).

Gene ID: 24409

NCBI Accession: NP_036705

UniProt: Q00959

Pathways: Synaptic Membrane, Regulation of long-term Neuronal Synaptic Plasticity