Solute Carrier Family 14 (Urea Transporter, Kidney) Member 2 (SLC14A2) (AA 911-929) antibody (HRP)

Catalog No. ABIN2486311

The Rabbit Polyclonal anti- antibody has been validated for WB, ICC, IF and IHC. It is suitable to detect in samples from Rat.

Quick Overview for Solute Carrier Family 14 (Urea Transporter, Kidney) Member 2 (SLC14A2) (AA 911-929) antibody (HRP) (ABIN2486311)

Target: Solute Carrier Family 14 (Urea Transporter, Kidney) Member 2 (SLC14A2)

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: HRP

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)

Product Details

Binding Specificity: AA 911-929

Specificity: Isoform 1 and isoform 2 are expressed in the inner medulla of the kidney. Isoform 3 and isoform 4 are expressed in both the inner and outer renal medulla.,Detects ~97 and 127 kDa.

Cross-Reactivity: Mouse, Rat

Purification: Peptide Affinity Purified

Immunogen: Produced against a synthetic peptide mapped to the C-terminal tail (amino acids 911-929) of rat UT-A1 (antibody designation L194)

Application Details

Application Notes:

• WB (1:1000)
• IHC (1:25)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN2486311 was sufficient for detection of UT-A1 in 20 μg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details

Target: Solute Carrier Family 14 (Urea Transporter, Kidney) Member 2 (SLC14A2)

Alternative Name: UT-A1

Background: UT-A1, a kidney-specific urea transporter is expressed in the renal collecting duct where it mediates trans-epithelial urea transport and is a target for regulation by vasopressin. Urea movement out of the collecting duct in the inner medulla of the kidney allows accumulation of urea in the medullary interstitium, thereby allowing maximum water reabsorption from the collecting ducts. (The antibody also recognizes a second protein from the UT-A gene driven by transcription from an alternative promoter and expressed in the thin descending limb of Henle, viz. UT-A2) (1, 2).

Gene ID: 54302

NCBI Accession: NP_062220

UniProt: Q62668

Pathways: Response to Water Deprivation