Integrin beta 2 antibody (AA 86-99)

Catalog No. ABIN2613445

This anti-Integrin beta 2 antibody is a Goat Polyclonal antibody detecting Integrin beta 2 in WB, IHC and ELISA. Suitable for Human.

Quick Overview for Integrin beta 2 antibody (AA 86-99) (ABIN2613445)

Target: Integrin beta 2 (ITGB2)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Integrin beta 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-Integrin beta 2 Antibody

Binding Specificity: AA 86-99

Purpose: ITGB2 / LFA-1 (aa86-99)

Sequence: ETQEDHNGGQ KQLS

Specificity: Reported variants represent identical protein: NP_001120963.1, NP_000202.2.

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Spleen. Recommended concentration: 5 μg/mL.

Western Blot: Approx 90 kDa band observed in lysates of cell lines K562 and U937 (calculated MW of 84.8 kDa according to NP_00202.2). Recommended concentration: 0.3-1 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Integrin beta 2

Target: Integrin beta 2 (ITGB2)

Alternative Name: ITGB2

Background: ITGB2, integrin, beta 2 (complement component 3 receptor 3 and 4 subunit), CD18, LAD, LCAMB, LFA-1, MAC-1, MF17, MFI7, cell surface adhesion glycoprotein LFA-1/CR3/P150,959 beta subunit precursor), cell surface adhesion glycoproteins LFA-1/CR3/p150,95 sub

Gene ID: 3689

NCBI Accession: NP_000202

Pathways: NF-kappaB Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation