NFATC3 antibody (Internal Region)

Catalog No. ABIN2613459

The Goat Polyclonal anti-NFATC3 antibody is suitable to detect NFATC3 in samples from Human. It has been validated for WB and ELISA.

Quick Overview for NFATC3 antibody (Internal Region) (ABIN2613459)

Target: NFATC3 (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 3 (NFATC3))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This NFATC3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-NFATC3 Antibody

Binding Specificity: Internal Region

Purpose: NFATC3 / NFAT4

Sequence: HQELDAHEDD LQIN

Specificity: This antibody is expected to recognize all four reported isoforms (NP_775188.1, NP_004546.1, NP_775186.1, NP_775187.1).

Cross-Reactivity: Cow, Dog, Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 150 kDa band observed in nuclear lysates of cell line Jurkat (calculated MW of 116 kDa according to NP_775188.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.03-0.1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for NFATC3

Target: NFATC3 (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 3 (NFATC3))

Alternative Name: NFATC3

Background: NFATC3, nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3, NFAT4, NFATX, T cell transcription factor NFAT4, nuclear factor of activated T-cells c3 isoform IE-Xa, nuclear factor of activated T-cells, cytoplasmic 3

Gene ID: 4775

NCBI Accession: NP_775188, NP_004546, NP_775186, NP_775187

Pathways: RTK Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, Chromatin Binding