ATM antibody (AA 2577-3056)

Catalog No. ABIN2617305

The Mouse Monoclonal anti-ATM antibody has been validated for WB, IHC, IF, IP and IHC (p). It is suitable to detect ATM in samples from Human, Mouse, Rat and Monkey.

Quick Overview for ATM antibody (AA 2577-3056) (ABIN2617305)

Target: ATM (Ataxia Telangiectasia Mutated (ATM))

Reactivity: Human, Mouse, Rat, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This ATM antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 2C1

Product Details anti-ATM Antibody

Binding Specificity: AA 2577-3056

Specificity: Recombinant protein expressed in E. coli corresponding to amino acids 2577-3056.

Purification: Protein G purified

Immunogen: Recombinant human ATM.

Type of Immunogen: Recombinant protein

Isotype: IgG1

Application Details

Application Notes: Approved: IF, IHC, IHC-P (5 μg/mL), IP, WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4. Preservative free.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for ATM

Target: ATM (Ataxia Telangiectasia Mutated (ATM))

Alternative Name: ATM

Background: Name/Gene ID: ATM
Subfamily: PI3/PI4
Family: Protein Kinase

Synonyms: ATM, A-T mutated, ATA, ATC, ATD, ATE, AT mutated, Ataxia telangiectasia, ATDC, Serine-protein kinase ATM, AT1, Ataxia telangiectasia mutated, TEL1, TELO1

Gene ID: 472

Pathways: p53 Signaling, Apoptosis, DNA Damage Repair, Inositol Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus