Caspase 8 antibody

Catalog No. ABIN2618019

The Rat Monoclonal anti-Caspase 8 antibody has been validated for WB, IHC, ICC, ELISA and FACS. It is suitable to detect Caspase 8 in samples from Mouse.

Quick Overview for Caspase 8 antibody (ABIN2618019)

Target: Caspase 8 (CASP8)

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Caspase 8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), ELISA, Flow Cytometry (FACS)

Clone: 3B10

Product Details anti-Caspase 8 Antibody

Specificity: Recognizes the p20 subunit of mouse caspase-8. Detects bands of ~55  kDa (full-length caspase-8) and ~18  kDa (apoptosis-induced cleavage fragment) by Western blot. Does not cross-react with human caspase-8.

Purification: Protein G purified from tissue culture supernatant

Immunogen: Recombinant mouse caspase-8 (p20 subunit).

Type of Immunogen: Recombinant protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA, Flo, ICC, IHC, WB (1 - 2 μg/mL)

Usage: ELISA - Recombinant protein. Flow Cytometry - Overexpressed in cell lines. Immunocytochemistry - Excellent, see literature reference 2. Western Blot - 1-2 μg/mL.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Caspase 8

Target: Caspase 8 (CASP8)

Alternative Name: CASP8 / Caspase 8

Background: Name/Gene ID: CASP8
Subfamily: Cysteine C14
Family: Protease

Synonyms: CASP8, ALPS2B, Apoptotic protease Mch-5, Apoptotic cysteine protease, Casp-8, Caspase-8, CAP4, Caspase 8, MACH, MCH5, MORT1-associated ced-3 homolog, ICE-like apoptotic protease 5, Pro Caspase 8, FADD-like ICE, FLICE, MACH-alpha-1/2/3 protein, MACH-beta-1/2/3/4 protein

Gene ID: 841

Pathways: Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Activation of Innate immune Response, Tube Formation, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades