H2AFX antibody (pSer139)

Catalog No. ABIN2621696

This Rabbit Monoclonal antibody specifically detects H2AFX in WB, IHC, IF and FACS. It exhibits reactivity toward Mouse, Rat and Monkey.

Quick Overview for H2AFX antibody (pSer139) (ABIN2621696)

Target: H2AFX (H2A Histone Family, Member X (H2AFX))

Reactivity: Mouse, Rat, Monkey

Host: Rabbit

Clonality: Monoclonal

Conjugate: This H2AFX antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: 20E3

Product Details anti-H2AFX Antibody

Binding Specificity: pSer139

Specificity: Recognizes endogenous levels of human H2A.X, phosphorylated at Ser139. Species Crossreactivity: Monkey, mouse, and rat

Purification: Purified

Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Ser139 of human H2A.X.

Type of Immunogen: Synthetic phospho-peptide

Isotype: IgG

Application Details

Application Notes: Approved: Flo (1:400 - ), IF (1:400 - ), IHC (1:400 - ), WB (1:1000 - )

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10  mM sodium HEPES,  pH 7.5, 150  mM sodium chloride, 100 μg/mL BSA, <0.02 % sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for 1 year.

Target Details for H2AFX

Target: H2AFX (H2A Histone Family, Member X (H2AFX))

Alternative Name: H2AFX / H2AX

Background: Name/Gene ID: H2AFX

Synonyms: H2AFX, H2AX, Histone H2A.x, H2A.X, H2A histone family, member X, H2AX histone

Gene ID: 3014

Pathways: Telomere Maintenance, DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus