Insulin antibody

Catalog No. ABIN2624251

This anti-Insulin antibody is a Guinea Pig Polyclonal antibody detecting Insulin in ELISA, IF and DB. Suitable for Pig.

Quick Overview for Insulin antibody (ABIN2624251)

Target: Insulin (INS)

Reactivity: Pig

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This Insulin antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF), Dot Blot (DB)

Product Details anti-Insulin Antibody

Specificity: Swine insulin

Purification: Purified IgG

Immunogen: Insulin is a hormone produced by the beta cells of the islets of Langerhans in the pancreas. It plays an important role in the metabolism of carbohydrate by its effects on the storage of glycogen in the liver, the conversion of carbohydrate to fat, and the oxidation of glucose by the peripheral tissues. Insulin has a molecular weight of 6,000 and contains two peptide chains linked by two disulphide bonds. The hormone is isolated from porcine pancreas. Freund's complete adjuvant is used in the fi

Type of Immunogen: Purified protein

Application Details

Application Notes: Approved: DB, ELISA, IF

Usage: The reactivity of the antiserum is directed to insulin as tested by ELISA and immunoblotting. No reaction was obtained with other swine serum proteins. The cytochemical grade allows the use in different types of highly sensitive immunoassays on appropriately treated cell and tissue substrates. in radioimmunoassay. for the production of immunoconjugates with a selected marker. to prepare immunoaffinity adsorbents by coupling to an artificial carrier. in non-isotopic methodology based on solid phase immunochemistry (e. g. ELISA), both as catching antibody and detection reagent. in Western blotting. This product is not pre-diluted. The optimum working dilution of each product should be established by titration before being used. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:250. in ELISA and comparable non-precipitating antibody-binding assays are between 1:500 and 1:5000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS, pH 7.2. No preservative added.

Preservative: Without preservative

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C
Short term: +4°C. Avoid repeat freeze-thaw cycles.

Target Details for Insulin

Target: Insulin (INS)

Alternative Name: Insulin

Background: Name/Gene ID: INS
Family: Hormone

Synonyms: INS, IRDN, IDDM2, Preproinsulin, ILPR, Insulin, MODY10, Proinsulin

Gene ID: 3630

Pathways: NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation