GCC2 antibody (Internal Region)

Catalog No. ABIN263163

This anti-GCC2 antibody is a Goat Polyclonal antibody detecting GCC2 in IHC and ELISA. Suitable for Human.

Quick Overview for GCC2 antibody (Internal Region) (ABIN263163)

Target: GCC2 (GRIP and Coiled-Coil Domain Containing 2 (GCC2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This GCC2 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-GCC2 Antibody

Binding Specificity: Internal Region

Purpose: GCC185

Sequence: QKLTNKNNKI ED

Specificity: This antibody is expected to recognise both reported isoforms (NP_055450.1and NP_852118.1).

Cross-Reactivity: Human, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QKLTNKNNKIED, from the internal region of the protein sequence according to NP_055450.1, NP_852118.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Spleen, Small Intestine and Kidney. Recommended concentration: 3.75 μg/mL.

Western Blot: Preliminary experiments gave an approx 60 kDa band in Human Lymph Node and Testis lysates after 0.03 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calcula

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for GCC2

Target: GCC2 (GRIP and Coiled-Coil Domain Containing 2 (GCC2))

Alternative Name: GCC2

Background: GCC2, GRIP and coiled-coil domain containing 2, GCC185, KIAA0336, CLL-associated antigen KW-11, CTCL tumor antigen se1-1, GRIP and coiled-coil domain-containing 2, GRIP coiled-coil protein GCC185, (Ran-binding protein 2-like 4)

Gene ID: 9648

NCBI Accession: NP_055450, NP_852118

Pathways: Maintenance of Protein Location, SARS-CoV-2 Protein Interactome