Cytokeratin 5 antibody

Catalog No. ABIN264415

This Mouse Monoclonal antibody specifically detects Cytokeratin 5 in WB and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for Cytokeratin 5 antibody (ABIN264415)

Target: Cytokeratin 5 (KRT5) (Keratin 5 (KRT5))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytokeratin 5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 16-04-14

Product Details anti-Cytokeratin 5 Antibody

Cross-Reactivity (Details): Also reacts to: KRT14

Characteristics: Synonyms: KRT5, Cytokeratin-5, Keratin-5, Keratin 5, CK5, K5, Keratin type II cytoskeletal 5, 58 kDaCytokeratin

Purification: Purified

Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with cytokeratinenriched extract of Feline tongue epithelium and Mouse myeloma cells.

Isotype: IgG2a

Application Details

Application Notes: Western Blot: Detects a 58 kDa band, corresponding to Keratin 5, and a 50 kDa band,corresponding to Keratin 14. Immunohistochemistry on Frozen Sections: 1-5 μg/mL. Does not work on Paraffin embedded tissue sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.4 mg/mL

Buffer: PBS containing 0.08 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C. Can be shipped at 2-8 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.

Expiry Date: 12 months

Target Details for Cytokeratin 5

Target: Cytokeratin 5 (KRT5) (Keratin 5 (KRT5))

Alternative Name: Cytokeratin 5

Background: Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non-keratinizing cells (ie., mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different cytokeratins (CK) in the molecular range of 40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification. Belonging to the type A (acidic) subfamily of low molecular weight keratins and existing in combination with keratin 5, keratin 14 distinguishes stratified epithelial cells from simple epithelial cells and is useful in identification of squamous cell carcinomas. It is considered a prognostic marker in breast carcinomas.Synonyms: 58 kDa Cytokeratin, CK5, Cytokeratin-5, K5, KRT5, Keratin 5, Keratin type II cytoskeletal 5, Keratin-5

Gene ID: 3852

UniProt: P13647