Adenovirus E1A antibody

Catalog No. ABIN264902

The Sheep Polyclonal anti-Adenovirus E1A antibody is suitable to detect Adenovirus E1A in samples from Adenovirus. It has been validated for IP.

Quick Overview for Adenovirus E1A antibody (ABIN264902)

Target: Adenovirus E1A (HAdV E1A) (Human Adenovirus E1A (HAdV E1A))

Reactivity: Adenovirus

Host: Sheep

Clonality: Polyclonal

Conjugate: This Adenovirus E1A antibody is un-conjugated

Application: Immunoprecipitation (IP)

Product Details anti-Adenovirus E1A Antibody

Purification: Purified

Immunogen: GST-EIA fusion protein recognizing a 43kD protein.

Isotype: IgG

Application Details

Application Notes: Western Blot: 1-10 μg /mL. Positive Control: 293 cells are human embryonic kidney cells that have been transformedwith the adenovirus genes E1A and E1B, which are required for adenovirus propagation. This cell line can be used as a positive control for this antibody.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: Phosphate buffered saline with 0.08 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for Adenovirus E1A

Target: Adenovirus E1A (HAdV E1A) (Human Adenovirus E1A (HAdV E1A))

Target Type: Viral Protein

Background: The early region (E1) of the adenovirus genome, responsible for transforming activity, is localized within the left most 11 % of the viral genome and consists of two transcriptional units E1A and E1B. E1A is sufficient for partial transformation and immortalization of primary cells. E1A gene products are necessary for normal levels of transcription of the other early regions of the adenovirus genome during productive infection and are able to either activate or repress the transcription of specific cellular genes. E1A forms specific complexes with cellular proteins including p105 causing inhibition of the cell cycle inducing arresting function of p105. EIA gene encodes potent onco-proteins that modify the normal transcriptional growth regulation of key cellular genes and thus alter cell cycle control.Synonyms: Adenovirus early region 1 antibody, Control protein E1A antibody, E1A antibody