Adenovirus E1A antibody

Catalog No. ABIN306547

This Sheep Polyclonal antibody specifically detects Adenovirus E1A in IP. It exhibits reactivity toward Human and Rat.

Quick Overview for Adenovirus E1A antibody (ABIN306547)

Target: Adenovirus E1A (HAdV E1A) (Human Adenovirus E1A (HAdV E1A))

Reactivity: Human, Rat

Host: Sheep

Clonality: Polyclonal

Conjugate: This Adenovirus E1A antibody is un-conjugated

Application: Immunoprecipitation (IP)

Product Details anti-Adenovirus E1A Antibody

Purpose: Sheep anti Human, Rat E1A (adenovirus early region 1)

Characteristics: The immunogen for the anti-EIA was a recombinant E1A protein recognizing a 43kD protein. EIA gene encodes potent onco-proteins that modify the normal transcriptional growth regulation of key cellular genes and thus alter cell cycle control. E1A protein has both transforming and trans-activating activities. Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle. Disrupts the function of host retinoblastoma protein RB1/pRb and isoform early E1A 26 kDa protein stabilizes TP53, which are key regulators of the cell cycle. Induces the disassembly of the E2F1 transcription factors from RB1 by direct competition for the same binding site on RB1, with subsequent transcriptional activation of E2F1-regulated S-phase genes. Inactivation of the ability of RB1 to arrest the cell cycle is critical for cellular transformation, uncontrolled cellular growth and proliferation induced by viral infection. Stimulation of progression from G1 to S phase allows the virus to efficiently use the cellular DNA replicating machinery to achieve viral genome replication. Interaction with RBX1 and CUL1 inhibits ubiquitination of the proteins targeted by SCF(FBW7) ubiquitin ligase complex, and may be linked to unregulated host cell proliferation. The tumorigenesis-restraining activity of E1A may be related to the disruption of the host CtBP-CtIP complex through the CtBP binding motif

Purification: Ammonium Sulfate Precipitation

Immunogen: Recombinant E1A protein recognizing a 43kD protein

Isotype: IgG

Application Details

Application Notes: Western Blot suggested dilution 1- 10 μg /mL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for Adenovirus E1A

Target: Adenovirus E1A (HAdV E1A) (Human Adenovirus E1A (HAdV E1A))

Alternative Name: E1A (adenovirus early region 1)

Target Type: Viral Protein

Background: E1A Early Adenovirus region 1

UniProt: P03254