Adenovirus E1A antibody

Catalog No. ABIN306558

The Mouse Monoclonal anti-Adenovirus E1A antibody (Clone M73) (ABIN306558) specifically detects Adenovirus E1A in IP and IF. The antibody is reactive with Human samples.

Quick Overview for Adenovirus E1A antibody (ABIN306558)

Target: Adenovirus E1A (HAdV E1A) (Human Adenovirus E1A (HAdV E1A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Adenovirus E1A antibody is un-conjugated

Application: Immunoprecipitation (IP), Immunofluorescence (IF)

Clone: M73

Product Details anti-Adenovirus E1A Antibody

Purpose: Mouse anti Human E1A (adenovirus early region 1)

Characteristics: The early region (E1) of the adenovirus genome, responsible for transforming activity, is localized within the left most 11 % of the viral genome and consists of two transcriptional units E1A and E1B. E1A protein has both transforming and trans-activating activities. Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle. Disrupts the function of host retinoblastoma protein RB1/pRb and isoform early E1A 26 kDa protein stabilizes TP53, which are key regulators of the cell cycle. Induces the disassembly of the E2F1 transcription factors from RB1 by direct competition for the same binding site on RB1, with subsequent transcriptional activation of E2F1-regulated S-phase genes. Inactivation of the ability of RB1 to arrest the cell cycle is critical for cellular transformation, uncontrolled cellular growth and proliferation induced by viral infection. Stimulation of progression from G1 to S phase allows the virus to efficiently use the cellular DNA replicating machinery to achieve viral genome replication. Interaction with RBX1 and CUL1 inhibits ubiquitination of the proteins targeted by SCF(FBW7) ubiquitin ligase complex, and may be linked to unregulated host cell proliferation. The tumorigenesis-restraining activity of E1A may be related to the disruption of the host CtBP-CtIP complex through the CtBP binding motif

Purification: Ammonium sulfate precipitation

Isotype: IgG2a

Application Details

Application Notes: Immunoprecipitation (1-2 μg/mL) and 2-5 μg/mL for Immunofluorescence.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for Adenovirus E1A

Target: Adenovirus E1A (HAdV E1A) (Human Adenovirus E1A (HAdV E1A))

Alternative Name: E1A (adenovirus early region 1)

Target Type: Viral Protein

Background: Mouse anti E1A, Adenovirus Early Region 1,

UniProt: P03254