CHEK1 antibody (AA 1-474)

Catalog No. ABIN264916

This Sheep Polyclonal antibody specifically detects CHEK1 in WB and IP. It exhibits reactivity toward Human, Mouse, Rat and Monkey.

Quick Overview for CHEK1 antibody (AA 1-474) (ABIN264916)

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Reactivity: Human, Mouse, Rat, Monkey

Host: Sheep

Clonality: Polyclonal

Conjugate: This CHEK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-CHEK1 Antibody

Binding Specificity: AA 1-474

Specificity: This antibody recognizes CHK1 kinase.

Purification: Purified

Immunogen: A GST fusion protein corresponding to amino acid sequence 1-474 with having a molecular weight of 54 kD.

Isotype: IgG

Application Details

Application Notes: Western Blot: 1-10 μg/mL. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: Phosphate buffered saline with 0.08 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for CHEK1

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Alternative Name: CHK1

Background: One mechanism by which checkpoints maintain the fidelity of cell cycle events is through blocking mitosis in response to unreplicated or damaged DNA. In most species this occurs through inhibiting activation of the cyclic-dependent kinase cdc2, which regulates entry in to mitosis. Chk1, a kinase involved in the DNA damage checkpoint response can phosphorylate Cdc25C an activator of cdc2. It is hypothesized that chk 1 induces serine 216 phosphorylation of Cdc 25C and subsequent binding negatively regulated Cdc25C, thus preventing it from activating cdc2.Synonyms: CHEK-1, CHEK1, CHK1 checkpoint homolog, Serine/threonine-protein kinase Chk1

Gene ID: 1111

NCBI Accession: NP_001107593

UniProt: O14757

Pathways: p53 Signaling, Apoptosis, Cell Division Cycle, DNA Damage Repair