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PRKDC antibody

PRKDC Reactivity: Human, Mouse WB, IF, IHC (p), EIA Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN265386
  • Target See all PRKDC Antibodies
    PRKDC (Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC))
    Reactivity
    • 161
    • 90
    • 49
    • 5
    • 2
    • 2
    • 2
    • 2
    • 1
    Human, Mouse
    Host
    • 153
    • 21
    • 3
    • 2
    Rabbit
    Clonality
    • 148
    • 31
    Polyclonal
    Conjugate
    • 95
    • 11
    • 11
    • 8
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    This PRKDC antibody is un-conjugated
    Application
    • 85
    • 69
    • 58
    • 35
    • 33
    • 28
    • 27
    • 24
    • 14
    • 13
    • 8
    • 7
    • 5
    • 4
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
    Specificity
    This antibody detects endogenous levels of DNA-PKcs protein (region surrounding Arg4090).
    Cross-Reactivity (Details)
    Species reactivity (expected):Mouse and Rat.
    Species reactivity (tested):Human.
    Purification
    The antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen
    Purity
    > 95 % pure by SDS-PAGE
    Top Product
    Discover our top product PRKDC Primary Antibody
  • Application Notes
    ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Buffer
    Phosphate buffered saline (PBS), pH 7.2., 0.05 % Sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target
    PRKDC (Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC))
    Alternative Name
    DNA-PKcs / PRKDC / XRCC7 (PRKDC Products)
    Synonyms
    DNA-PKcs antibody, DNAPK antibody, DNPK1 antibody, HYRC antibody, HYRC1 antibody, XRCC7 antibody, p350 antibody, DNA-PKCS antibody, dna-pkcs antibody, dnapk antibody, dnpk1 antibody, hyrc antibody, hyrc1 antibody, prkdc-A antibody, xrcc7 antibody, PRKDC antibody, AI326420 antibody, AU019811 antibody, DNAPDcs antibody, DOXNPH antibody, dxnph antibody, p460 antibody, scid antibody, slip antibody, protein kinase, DNA-activated, catalytic polypeptide antibody, protein kinase, DNA-activated, catalytic polypeptide L homeolog antibody, DNA-dependent protein kinase catalytic subunit antibody, protein kinase, DNA activated, catalytic polypeptide antibody, PRKDC antibody, prkdc.L antibody, LOC100633457 antibody, Prkdc antibody
    Background
    The phosphatidylinositol kinase (PIK) family members fall into two distinct subgroups. The first subgroup contains proteins such as the PI 3- and PI 4- kinases and the second group comprises the PIK-related kinases. The PIKrelated kinases include Atm, DNA-PKCS and FRAP. These proteins have in common a region of homology at their carboxy termini that is not present in the PI 3- and PI 4-kinases. The Atm gene is mutated in the autosomal recessive disorder ataxia telangiectasia (AT) that is characterized by cerebellar degeneration (ataxia) and the appearance of dilated blood vessels (telangiectases) in the conjunctivae of the eyes. AT cells are hypersensitive to ionizing radiation, impaired in mediating the inhibition of DNA synthesis and they display delays in p53 induction. DNA-PK is a heterotrimeric DNA binding enzyme that is composed of a large subunit, DNA-PKCS, and two smaller subunits collectively known as Ku. The loss of DNA-PK leads to defects in DSB repair and V(D)J recombination. FRAP can autophosphorylate on serine and bind to Rapamycin/FKBP. FRAP is also an upstream regulator of S6 kinase and has been implicated in the regulation of p27 and p21 expression.Synonyms: DNA-dependent protein kinase catalytic subunit, DNPK1, HYRC, HYRC1, p460
    Molecular Weight
    approx. 450 kDa
    Gene ID
    5591
    NCBI Accession
    NP_001075109
    UniProt
    P78527
    Pathways
    DNA Damage Repair, Production of Molecular Mediator of Immune Response
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