KRT15 antibody

Catalog No. ABIN265494

Product Details anti-KRT15 Antibody

Target: KRT15 (Keratin 15 (KRT15))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KRT15 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of Keratin 15 protein. (region surrounding Thr12)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity Chromatography using epitope-specific immunogen.

Application Details

Application Notes: ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for KRT15

Target: KRT15 (Keratin 15 (KRT15))

Alternative Name: Cytokeratin 15 (KRT15 Products)

Synonyms: xlk2 antibody, CK15 antibody, K15 antibody, K1CO antibody, AI528832 antibody, Krt1-15 antibody, Ka15 antibody, wu:fa91c06 antibody, wu:fa95d04 antibody, zgc:55334 antibody, keratin 15, type I L homeolog antibody, keratin 15, type I antibody, keratin 15 antibody, krt15.L antibody, krt15 antibody, KRT15 antibody, Krt15 antibody

Background: Cytokeratin 15 (CK15, K15, K1CO, keratin15, type I cytoskeletal 15) is an intermediate filament (IF) type I protein that is responsible for the mechanical integrity of epithelial cells. Keratin family members are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains, and are clustered in a region on chromosome 17q21.2. Cytokeratin 15 is a specific marker of stem cells of the hair-follicle bulge and may be a useful marker for diagnosis between basal cell carcinoma and trichoepithelioma. Trichoblastoma are benign neoplasms of follicular differentiation frequently found in nevus sebaceus. Many morphologic features are shared with nodular basal cell carcinoma, sometimes rendering a diagnosis difficult. Trichoblastoma and BCC show variable expression of Cytokeratin 15 and Cytokeratin 19, and absence of hair keratins.Synonyms: CK-15, Cytokeratin-15, K15, KRT15, KRTB, Keratin, Keratin 15, Keratin-15, Type I Cytoskeletal 15

Molecular Weight: approx. 49 kDa

Gene ID: 3866

NCBI Accession: NP_002266

UniProt: P19012