ITGA4 antibody (APC)

Catalog No. ABIN2658679

The Mouse Monoclonal anti-ITGA4 antibody is suitable to detect ITGA4 in samples from Human. It has been validated for FACS and ICFC.

Quick Overview for ITGA4 antibody (APC) (ABIN2658679)

Target: ITGA4 (Integrin alpha 4 (ITGA4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ITGA4 antibody is conjugated to APC

Application: Flow Cytometry (FACS), Intracellular Flow Cytometry (ICFC)

Clone: 9F10

Product Details anti-ITGA4 Antibody (APC)

Cross-Reactivity: Baboon, Chimpanzee, Marmoset, Cynomolgus, Rhesus Monkey, Squirrel Monkey (Saimiri spec.), Horse (Equine), Cow (Bovine), Sheep (Ovine), Dog (Canine), Cat (Feline)

Purification: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 0.2 % (w/v) BSA .

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from prolonged exposure to light. Do not freeze.

Storage: 4 °C

Storage Comment: The antibody solution should be stored undiluted between 2°C and 8°C.

Target Details for ITGA4

Target: ITGA4 (Integrin alpha 4 (ITGA4))

Alternative Name: CD49d

Background: CD49d is a 150 kD α integrin chain known as α4 integrin or VLA-4 α chain. It forms a heterodimer with either integrin β1 (α4β1, VLA-4) or β7 (α4β7). CD49d is expressed broadly on T lymphocytes, B lymphocytes, monocytes, thymocytes, eosinophils, basophils, mast cells, NK cells, dendritic cells, and some non-hematopoietic cells, but not on normal red blood cells, platelets or neutrophils. VLA-4 binds to VCAM-1 (CD106) and fibronectin. α4β7 is the receptor for VCAM-1 and MAdCAM-1. CD49d participates in mononuclear cell trafficking to endothelial sites of inflammation and has roles in cell-cell interactions and cell adhesion to extracellular matrices. CD49d is involved in lymphocyte migration, T cell activation, and hematopoietic stem cell differentiation. CD49d is a marker to isolate pure populations of Treg cells due to its absence on Foxp3+ cells.

Pathways: Integrin Complex