H2AFX antibody (pSer139)

Catalog No. ABIN2666075

The Mouse Monoclonal anti-H2AFX antibody (Clone 2F3) (ABIN2666075) specifically detects H2AFX in BCA. The antibody is reactive with Human and Mouse samples.

Quick Overview for H2AFX antibody (pSer139) (ABIN2666075)

Target: H2AFX (H2A Histone Family, Member X (H2AFX))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This H2AFX antibody is un-conjugated

Application: Biochemical Assay (BCA)

Clone: 2F3

Product Details anti-H2AFX Antibody

Binding Specificity: pSer139

Purification: The antibody was purified by affinity chromatography.

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide. Final antibody concentration is 0.5 mg/mL.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Upon receipt, store between 2°C and 8°C.

Target Details for H2AFX

Target: H2AFX (H2A Histone Family, Member X (H2AFX))

Alternative Name: H2A.X

Background: H2A.X is a 14 kD basal histone and a member of the H2 histone family. This nuclear protein is synthesized in the G1 and S phase of the cell cycle and is known to be important for recombination between immunoglobulin switch regions. H2A.X becomes phosphorylated on serine 139 after double-stranded DNA breaks. Phosphorylated H2A.X promotes DNA repair and maintains genomic stability. The 2F3 monoclonal antibody reacts with phosphorylated human H2A.X (Ser139) and has been shown to be useful for Western blotting and immunofluorescence.

Pathways: Telomere Maintenance, DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus