PRKDC antibody (phosphorylated)

Catalog No. ABIN2666330

The Mouse Monoclonal anti-PRKDC antibody (Clone 10B1) (ABIN2666330) specifically detects PRKDC in FACS. The antibody is reactive with Human samples.

Quick Overview for PRKDC antibody (phosphorylated) (ABIN2666330)

Target: PRKDC (Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PRKDC antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: 10B1

Product Details anti-PRKDC Antibody

Binding Specificity: phosphorylated

Cross-Reactivity (Details): reacts with Thr2609-phosphorylated DNA-PKcs

Purification: The antibody was purified by affinity chromatography.

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide. Final antibody concentration is 0.5 mg/mL.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Upon receipt, store undiluted between 2°C and 8°C.

Target Details for PRKDC

Target: PRKDC (Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC))

Alternative Name: DNA-PKcs

Background: DNA-protein kinase catalytic subunit (DNA-PKcs) also known as DNA-activated kinase is a nuclear 460-470 kD serine threonine kinase involved in double-stranded DNA break repair, VDJ recombination, and transcriptional modulation. DNA-PKcs must bind DNA ends to become active. DNA-PKcs is modified by phosphorylation and has been shown to interact with Ku70/Ku80, KIP, DNA-ligase IV, and XRCC4 proteins. Phosphorylated DNA-PKcs is upregulated after DNA damaage. The 10B1 monoclonal antibody recognizes phosphorylated human DNA-PKcs (Thr2609) and has been shown to be useful for immunofluorescence staining and Western blotting.

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response