Retinoblastoma Binding Protein 8 antibody (AA 620-897)

Catalog No. ABIN7938099

This Mouse Monoclonal antibody specifically detects Retinoblastoma Binding Protein 8 in WB, IF, IP, ICC and ChIP. It exhibits reactivity toward Human. It has been mentioned in 6+ publications

Quick Overview for Retinoblastoma Binding Protein 8 antibody (AA 620-897) (ABIN7938099)

Target: Retinoblastoma Binding Protein 8 (RBBP8)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Retinoblastoma Binding Protein 8 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP)

Clone: 14-1

Product Details anti-Retinoblastoma Binding Protein 8 Antibody

Binding Specificity: AA 620-897

Purpose: CtIP antibody (mAb) (Clone 14-1)

Purification: Protein A Chromatography

Immunogen: This CtIP antibody was raised against a recombinant protein corresponding to amino acid residues 620-897 of human CtIP.

Isotype: IgG1

Application Details

Application Notes: WB: 1 - 2 μg/mL

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-Retinoblastoma Binding Protein 8 antibody (ABIN7938099)

Onyango, Howard, Neherin, Yanez, Stark: "Tetratricopeptide repeat factor XAB2 mediates the end resection step of homologous recombination." in: Nucleic acids research, Vol. 44, Issue 12, pp. 5702-16, (2016) (PubMed).

Bakr, Köcher, Volquardsen, Petersen, Borgmann, Dikomey, Rothkamm, Mansour: "Impaired 53BP1/RIF1 DSB mediated end-protection stimulates CtIP-dependent end resection and switches the repair to PARP1-dependent end joining in G1." in: Oncotarget, Vol. 7, Issue 36, pp. 57679-57693, (2016) (PubMed).

Zhou, Caron, Legube, Paull: "Quantitation of DNA double-strand break resection intermediates in human cells." in: Nucleic acids research, Vol. 42, Issue 3, pp. e19, (2014) (PubMed).

Makharashvili, Tubbs, Yang, Wang, Barton, Zhou, Deshpande, Lee, Lobrich, Sleckman, Wu, Paull: "Catalytic and noncatalytic roles of the CtIP endonuclease in double-strand break end resection." in: Molecular cell, Vol. 54, Issue 6, pp. 1022-33, (2014) (PubMed).

Zhang, Jasin: "An essential role for CtIP in chromosomal translocation formation through an alternative end-joining pathway." in: Nature structural & molecular biology, Vol. 18, Issue 1, pp. 80-4, (2011) (PubMed).

Yu, Fu, Lai, Baer, Chen: "BRCA1 ubiquitinates its phosphorylation-dependent binding partner CtIP." in: Genes & development, Vol. 20, Issue 13, pp. 1721-6, (2006) (PubMed).

Target Details for Retinoblastoma Binding Protein 8

Target: Retinoblastoma Binding Protein 8 (RBBP8)

Alternative Name: CtIP

Background: CtIP (CTBP interacting protein, RBBP8, RIM, COM1) is a transcriptional regulator and tumor suppressor first identified through its interaction with CTBP (C-Terminal Binding Protein). CtIP binds to the Rb protein and serves as a transcriptional repressor, through recruitment of CtBP and also by interaction with general transcription factors TFIID and TFIIB. CtIP also interacts with Rb-related protein p130 and Ikaros, a master regulator in lymphocyte development. CtIP is phosphorylated upon DNA damage (likely by ATM and/or ATR), interacts with BRCA1 and plays a critical role in facilitating DNA damage repair. Mutations that abolish the interaction between CtIP and BRCA1 result in loss of cell cycle control leading to the development of cancer.

Molecular Weight: 120 kDa

Gene ID: 5932

NCBI Accession: NP_002885

Pathways: Cell Division Cycle, DNA Damage Repair