HIPK2 antibody (AA 898-1051)

Catalog No. ABIN2668288

There is 1 publication for this product available. The Mouse Monoclonal anti-HIPK2 antibody is suitable to detect HIPK2 in samples from Human. It has been validated for WB, IHC, IP, ICC and IF.

Quick Overview for HIPK2 antibody (AA 898-1051) (ABIN2668288)

Target: HIPK2 (Homeodomain Interacting Protein Kinase 2 (HIPK2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HIPK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: 3E9

Product Details anti-HIPK2 Antibody

Binding Specificity: AA 898-1051

Purpose: HIPK2 antibody (mAb) (Clone 3E9)

Purification: Protein G Chromatography

Immunogen: This HIPK2 antibody was raised against a recombinant protein including amino acids 898-1051 of mouse HIPK2.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-HIPK2 antibody (ABIN2668288)

Isono, Nemoto, Li, Takada, Suzuki, Katsuki, Nakagawara, Koseki: "Overlapping roles for homeodomain-interacting protein kinases hipk1 and hipk2 in the mediation of cell growth in response to morphogenetic and genotoxic signals." in: Molecular and cellular biology, Vol. 26, Issue 7, pp. 2758-71, (2006) (PubMed).

Target Details for HIPK2

Target: HIPK2 (Homeodomain Interacting Protein Kinase 2 (HIPK2))

Alternative Name: HIPK2

Background: HIPK2 (Homeodomain Interacting Protein Kinase 2) is a serine/threonine kinase present in the nucleus that interacts with homeodomain transcription factors and acts as a co-repressor of transcription. It inhibits cell growth, as well as promotes apoptosis through the phosphorylation and subsequent degradation of the anti-apoptotic factor CTBP1. HIPK2 also phosphorylates CBX4 upon DNA damage and promotes its E3 SUMO-protein ligase activity.

Molecular Weight: 130 kDa

Gene ID: 28996

NCBI Accession: NP_034563

Pathways: Cell Division Cycle