POLR2B antibody (N-Term)

Catalog No. ABIN2668294

The Rabbit Polyclonal anti-POLR2B antibody is suitable to detect POLR2B in samples from Human. It has been validated for WB and IP.

Quick Overview for POLR2B antibody (N-Term) (ABIN2668294)

Target: POLR2B (Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR2B antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-POLR2B Antibody

Binding Specificity: N-Term

Purpose: POLR2B antibody (pAb)

Purification: Affinity Purified

Immunogen: This antibody was raised against a peptide within the N-terminal region of human POLR2B.

Isotype: IgG

Application Details

Application Notes: IP: 5 - 10 µL per IP WB*: 1:500 - 1:1,000 dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for POLR2B

Target: POLR2B (Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B))

Alternative Name: POLR2B

Background: POLR2B (polymerase (RNA) II (DNA directed) polypeptide B, 140 kDa) DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB2 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template (By similarity).

Molecular Weight: 135 kDa

Gene ID: 5431

NCBI Accession: NP_000929

Pathways: Regulatory RNA Pathways, DNA Damage Repair