Histone H2B antibody (H2BS14ph)

Catalog No. ABIN2668331

Cited in 1 publication. The Mouse Monoclonal anti-Histone H2B antibody (Clone MABI 0251) (ABIN2668331) specifically detects Histone H2B in WB, IF and ICC. The antibody is reactive with Human samples.

Quick Overview for Histone H2B antibody (H2BS14ph) (ABIN2668331)

Target: Histone H2B

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Histone H2B antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: MABI 0251

Product Details anti-Histone H2B Antibody

Binding Specificity: H2BS14ph

Purpose: Histone H2BS14ph antibody (mAb) (Clone MABI 0251)

Purification: Protein G Chromatography

Immunogen: This Histone H2B phospho Ser14 antibody was raised against a peptide containing phospho Ser14 of human histone H2B.

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.46 μg/μL

Buffer: PBS pH 7.5 containing 30 % glycerol, 0.3M NaCl and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-Histone H2B antibody (ABIN2668331)

Kimura, Takizawa, Allemand, Hori, Iborra, Nozaki, Muraki, Hagiwara, Krainer, Fukagawa, Okawa: "A novel histone exchange factor, protein phosphatase 2Cgamma, mediates the exchange and dephosphorylation of H2A-H2B." in: The Journal of cell biology, Vol. 175, Issue 3, pp. 389-400, (2006) (PubMed).

Target Details for Histone H2B

Target: Histone H2B

Background: Histone H2B is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins. H2B is phosphorylated at serine 14 in response to DNA damage (dependant upon PI3 kinase kinase activity) and during apoptosis.by the Mst1 kinase.

Molecular Weight: 15 kDa

Gene ID: 8348

NCBI Accession: NP_778225