Histone H2B antibody (AA 111-125)

Catalog No. ABIN2668332

This Rabbit Polyclonal antibody specifically detects Histone H2B in WB. It exhibits reactivity toward Human.It has been mentioned in 1 publication

Quick Overview for Histone H2B antibody (AA 111-125) (ABIN2668332)

Target: Histone H2B

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H2B antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Histone H2B Antibody

Binding Specificity: AA 111-125

Purpose: Histone H2B antibody (pAb)

Purification: Protein G Chromatography

Immunogen: This Histone H2B antibody was raised against a peptide corresponding to amino acid residues 111-125 of human Histone H2B.

Isotype: IgG

Application Details

Application Notes: WB: 2 μg/mL dilution For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1 % Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required.

Restrictions: For Research Use only

Handling

Concentration: 0.5 μg/μL

Buffer: PBS containing 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: 4 °C,-20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Store at 4°C for short term. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-Histone H2B antibody (ABIN2668332)

Chandrasekharan, Huang, Chen, Sun: "Histone H2B C-terminal helix mediates trans-histone H3K4 methylation independent of H2B ubiquitination." in: Molecular and cellular biology, Vol. 30, Issue 13, pp. 3216-32, (2010) (PubMed).

Target Details for Histone H2B

Target: Histone H2B

Background: Histone H2B is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, they play a major role in regulating gene expression.

Molecular Weight: 15 kDa

Gene ID: 8348

NCBI Accession: NP_778225