Histone H2B antibody (H2BK46me2)

Catalog No. ABIN2668335

This Rabbit Polyclonal antibody specifically detects Histone H2B in WB, IF, ICC and DB. It exhibits reactivity toward Human.It has been mentioned in 1 publication

Quick Overview for Histone H2B antibody (H2BK46me2) (ABIN2668335)

Target: Histone H2B

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H2B antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Dot Blot (DB)

Product Details anti-Histone H2B Antibody

Binding Specificity: H2BK46me2

Purpose: Histone H2BK46me2 antibody (pAb)

Purification: Unpurified

Immunogen: This Histone H2B dimethyl Lys46 antibody was raised against a peptide containing dimethyl-lysine 46 of human histone H2B.

Application Details

Application Notes: WB: 1:500 - 1:2,000 dilution ICC/IF: 1:250 - 1:1,000 dilution

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit serum containing 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-Histone H2B antibody (ABIN2668335)

Kyriss, Jin, Gallegos, Sanford, Wyrick: "Novel functional residues in the core domain of histone H2B regulate yeast gene expression and silencing and affect the response to DNA damage." in: Molecular and cellular biology, Vol. 30, Issue 14, pp. 3503-18, (2010) (PubMed).

Target Details for Histone H2B

Target: Histone H2B

Background: Histone H2B is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, they play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Changes in methylation of histone H2B may be involved in heat-shock mechanisms.

Molecular Weight: 15 kDa

Gene ID: 8348

NCBI Accession: NP_003512