Histone H2B antibody (H2BK16ac)

Catalog No. ABIN2668339

There is 1 publication for this product available. The Rabbit Polyclonal anti-Histone H2B antibody is suitable to detect Histone H2B in samples from Human. It has been validated for WB, IF, ChIP and DB.

Quick Overview for Histone H2B antibody (H2BK16ac) (ABIN2668339)

Target: Histone H2B

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H2B antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB)

Product Details anti-Histone H2B Antibody

Binding Specificity: H2BK16ac

Purpose: Histone H2BK16ac antibody (pAb)

Purification: Unpurified

Immunogen: This Histone H2B acetyl Lys16 antibody was raised against a peptide including acetyl-lysine 16 of human histone H2B.

Application Details

Application Notes: ChIP: 5 - 10 µL per ChIP WB*: 1:5,000 - 1:20,000 dilution IF: 1:500 dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit serum containing 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-Histone H2B antibody (ABIN2668339)

Gatta, Mantovani: "Single nucleosome ChIPs identify an extensive switch of acetyl marks on cell cycle promoters." in: Cell cycle (Georgetown, Tex.), Vol. 9, Issue 11, pp. 2149-59, (2011) (PubMed).

Target Details for Histone H2B

Target: Histone H2B

Background: Histone H2B is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, they play a major role in regulating gene expression. Lysine N-e-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Histone H2A and Histone H2B are acetylated in bulk chromatin by p300 and form acetylated Histone H2A/Histone H2B heterodimers. When DNA associates with intact core histone octamers that contain acetylated H2A/H2B dimers, the inhibition of transcriptional initiation significantly decreases, indicating that acetylation of their lysine residues may mediate transcription. The acetylation of Histone H2B at Lys16 is associated with transcriptional activation and is involved in cell survival process.

Molecular Weight: 15 kDa

Gene ID: 8348

NCBI Accession: NP_778225