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CRISPR-Cas9 (N-Term) antibody Primary Antibody

Reactivity: Streptococcus pyogenes ICC, IF, IP, WB Host: Mouse Monoclonal 7A9-3A3 unconjugated
Catalog No. ABIN2668367
$530.83
Plus shipping costs $45.00
100 μg ABIN2668367
100 μg ABIN2668367
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  • Target
    CRISPR-Cas9
    Binding Specificity
    N-Term
    • 7
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    Reactivity
    Streptococcus pyogenes
    • 18
    • 5
    • 2
    • 1
    • 1
    Host
    Mouse
    Clonality
    Monoclonal
    Conjugate
    Un-conjugated
    • 20
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Application
    Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
    • 24
    • 10
    • 7
    • 5
    • 5
    • 5
    • 2
    • 2
    • 1
    Purification
    Protein G Chromatography
    Immunogen
    This antibody was raised against a recombinant protein within the N-terminal region of Streptococcus pyogene Cas9. This antibody should recognize Cas9 and dCas9 based on the antigen design.
    Clone
    7A9-3A3
    Isotype
    IgG1 kappa
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Concentration
    1 μg/μL
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice

    Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

    Storage
    -20 °C
    Storage Comment
    Antibodies in solution can be stored at -20 °C for 2 years.
    Expiry Date
    6 months
  • Target
    CRISPR-Cas9
    Alternative Name
    Cas9 ()
    Background
    Cas9 is a nuclease from Streptococcus pyogenes that can be targeted to particular DNA sequences through a guide RNA that results in double-stranded breaks in DNA. Cas9 is part of the CRISPR/Cas9 gene-editing system that can create a DNA break at a specific location with the genome. CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) Probable. In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed by 3'-5' exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif) to help distinguish self versus nonself.
    Molecular Weight
    160 kDa
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