DNMT1 antibody (AA 1-110)

Catalog No. ABIN2668501

There is 1 publication for this product available. The Rabbit Polyclonal anti-DNMT1 antibody is suitable to detect DNMT1 in samples from Human. It has been validated for WB, IP and IF.

Quick Overview for DNMT1 antibody (AA 1-110) (ABIN2668501)

Target: DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DNMT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)

Product Details anti-DNMT1 Antibody

Binding Specificity: AA 1-110

Purpose: DNMT1 antibody (pAb)

Purification: Protein A Chromatography

Immunogen: This DNMT1 antibody was raised against a recombinant protein corresponding to amino acids 1-110 of human DNMT1.

Isotype: IgG

Application Details

Application Notes: IP: 10 μg per IP WB*: 0.5 - 2 μg/mL dilution IF: 1:500 dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot. For DNMT1, we also offer AbFlex DNMT1 Recombinant Antibody (rAb). For details, see Catalog No. 91175.

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-DNMT1 antibody (ABIN2668501)

Londoño Gentile, Lu, Lodato, Tse, Olejniczak, Witze, Thompson, Wellen: "DNMT1 is regulated by ATP-citrate lyase and maintains methylation patterns during adipocyte differentiation." in: Molecular and cellular biology, Vol. 33, Issue 19, pp. 3864-78, (2013) (PubMed).

Target Details for DNMT1

Target: DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

Alternative Name: DNMT1

Background: DNMT1 (DNA (cytosine-5)-methyltransferase) proteins are involved in DNA methylation in which a methyl group is added to a cytosine residue on DNA, commonly at the C5 position of a CpG dinucleotide. Three families of DNMTs have been identified: DNMT1, DNMT2, and DNMT3 (comprised of DNMT3A and DNMT3B). DNMT1 is the most abundant DNMT in somatic cells and shows a preference for methylating hemi-methylated DNA. It is considered to be a maintenance DNA methyltransferase that is important in the maintenance of specific patterns of methylation throughout cellular divisions. Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as embryonic development, genetic imprinting, X chromosome inactivation and the control of gene expression. DNA methylation is generally associated with transcriptional repression.

Molecular Weight: 185 kDa

Gene ID: 1786

NCBI Accession: NP_001370

Pathways: SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection