DNMT1 antibody

Catalog No. ABIN2668503

This Rat Monoclonal antibody specifically detects DNMT1 in WB, IP and ChIP. It exhibits reactivity toward Human.It has been mentioned in 1 publication

Quick Overview for DNMT1 antibody (ABIN2668503)

Target: DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This DNMT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP)

Clone: 2C1

Product Details anti-DNMT1 Antibody

Purpose: DNMT1 antibody (mAb) (Clone 2C1)

Purification: Protein G Chromatography

Immunogen: This antibody was raised against a full-length recombinant protein corresponding to human DNMT1.

Isotype: IgG2b

Application Details

Application Notes: This antibody is also available as an AbFlex engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91175.

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-DNMT1 antibody (ABIN2668503)

Felle, Joppien, Németh, Diermeier, Thalhammer, Dobner, Kremmer, Kappler, Längst: "The USP7/Dnmt1 complex stimulates the DNA methylation activity of Dnmt1 and regulates the stability of UHRF1." in: Nucleic acids research, Vol. 39, Issue 19, pp. 8355-65, (2011) (PubMed).

Target Details for DNMT1

Target: DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

Alternative Name: DNMT1

Background: DNMT1 (DNA (cytosine-5)-methyltransferase) proteins are involved in DNA methylation in which a methyl group is added to a cytosine residue on DNA, commonly at the C5 position of a CpG dinucleotide. Three families of DNMTs have been identified: DNMT1, DNMT2, and DNMT3 (comprised of DNMT3A and DNMT3B). DNMT1 is the most abundant DNMT in somatic cells and shows a preference for methylating hemi-methylated DNA. It is considered to be a maintenance DNA methyltransferase that is important in the maintenance of specific patterns of methylation throughout cellular divisions. Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as embryonic development, genetic imprinting, X chromosome inactivation and the control of gene expression. DNA methylation is generally associated with transcriptional repression.

Molecular Weight: 190 kDa

Gene ID: 1786

NCBI Accession: NP_001124295

Pathways: SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection