Histone H2B antibody (H2BK120ub)

Catalog No. ABIN2668551

This Mouse Monoclonal antibody specifically detects Histone H2B in WB and ChIP. It exhibits reactivity toward Human. It has been mentioned in 3+ publications

Quick Overview for Histone H2B antibody (H2BK120ub) (ABIN2668551)

Target: Histone H2B

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Histone H2B antibody is un-conjugated

Application: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP)

Clone: 56

Product Details anti-Histone H2B Antibody

Binding Specificity: H2BK120ub

Purpose: Histone H2BK120ub1 antibody (mAb) (Clone 56)

Purification: Protein G Chromatography

Immunogen: This Histone H2B ubiquityl Lys120 antibody was raised against a branched peptide corresponding the the ubiquitin conjugation site at Lys120 of human Histone H2B.

Isotype: IgG2a

Application Details

Application Notes: WB*: 0.5 - 2 μg/mL dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-Histone H2B antibody (ABIN2668551)

Materne, Vázquez, Sánchez, Yague-Sanz, Anandhakumar, Migeot, Antequera, Hermand: "Histone H2B ubiquitylation represses gametogenesis by opposing RSC-dependent chromatin remodeling at the ste11 master regulator locus." in: eLife, Vol. 5, (2016) (PubMed).

Mbogning, Nagy, Pagé, Schwer, Shuman, Fisher, Tanny: "The PAF complex and Prf1/Rtf1 delineate distinct Cdk9-dependent pathways regulating transcription elongation in fission yeast." in: PLoS genetics, Vol. 9, Issue 12, pp. e1004029, (2014) (PubMed).

DeGennaro, Alver, Marguerat, Stepanova, Davis, Bähler, Park, Winston: "Spt6 regulates intragenic and antisense transcription, nucleosome positioning, and histone modifications genome-wide in fission yeast." in: Molecular and cellular biology, Vol. 33, Issue 24, pp. 4779-92, (2013) (PubMed).

Target Details for Histone H2B

Target: Histone H2B

Background: Histone H2B is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, they play a major role in regulating gene expression. Histone H2B is ubiquitylated at lysine 120 in vertebrates. H2B ubiquityl Lys120 is found preferentially associated with the transcribed regions of highly expressed genes. It is possible that H2B ubiquitylation is associated with transcriptional elongation.

Molecular Weight: 26 kDa

Gene ID: 8348

NCBI Accession: NP_003512