ASCL1 antibody

Catalog No. ABIN2668587

This Mouse Monoclonal antibody specifically detects ASCL1 in WB and IHC. It exhibits reactivity toward Mouse and Rat. It has been mentioned in 3+ publications

Quick Overview for ASCL1 antibody (ABIN2668587)

Target: ASCL1 (Achaete-Scute Complex Homolog 1 (Drosophila) (ASCL1))

Reactivity: Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This ASCL1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: 24B7-2D11-1

Product Details anti-ASCL1 Antibody

Purpose: MASH1 antibody (mAb) (Clone 24B7.2D11.1)

Purification: Protein A Chromatography

Immunogen: This MASH1 antibody was raised against a full-length protein corresponding to rat MASH1.

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-ASCL1 antibody (ABIN2668587)

Ivaniutsin, Chen, Mason, Price, Pratt: "Adenomatous polyposis coli is required for early events in the normal growth and differentiation of the developing cerebral cortex." in: Neural development, Vol. 4, Issue 1, pp. 3, (2009) (PubMed).

Ralston, Chiriboga, Nonaka: "MASH1: a useful marker in differentiating pulmonary small cell carcinoma from Merkel cell carcinoma." in: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, Vol. 21, Issue 11, pp. 1357-62, (2008) (PubMed).

Lo, Johnson, Wuenschell, Saito, Anderson: "Mammalian achaete-scute homolog 1 is transiently expressed by spatially restricted subsets of early neuroepithelial and neural crest cells." in: Genes & development, Vol. 5, Issue 9, pp. 1524-37, (1991) (PubMed).

Target Details for ASCL1

Target: ASCL1 (Achaete-Scute Complex Homolog 1 (Drosophila) (ASCL1))

Alternative Name: MASH1

Background: The MASH1 (achaete-scute complex homolog 1 (Drosophila), ASCL1, ASH1, HASH1) gene encodes a protein that is a basic helix-loop-helix transcription factor that may play a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS. Essential for the generation of olfactory and autonomic neurons. Involved in the initiation of neuronal differentiation. Mediates transcriptional activation by binding to the E box motif.

Molecular Weight: 34 kDa

Gene ID: 64186

NCBI Accession: NP_071779

Pathways: Dopaminergic Neurogenesis