MBD3 antibody

Catalog No. ABIN2668686

This Mouse Monoclonal antibody specifically detects MBD3 in WB. It exhibits reactivity toward Human.

Quick Overview for MBD3 antibody (ABIN2668686)

Target: MBD3 (Methyl-CpG Binding Domain Protein 3 (MBD3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MBD3 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 106B691

Product Details anti-MBD3 Antibody

Purpose: MBD3 antibody (mAb) (Clone 106B691)

Purification: Affinity Purified

Immunogen: This MBD3 antibody was raised against a synthetic peptide corresponding to human MBD3.

Isotype: IgG

Application Details

Application Notes: WB: 1 - 2 μg/mL dilution For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1 % Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required.

Restrictions: For Research Use only

Handling

Concentration: 0.5 μg/μL

Buffer: PBS containing 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: 4 °C,-20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Store at 4°C for short term. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for MBD3

Target: MBD3 (Methyl-CpG Binding Domain Protein 3 (MBD3))

Alternative Name: MBD3

Background: MBD3 (methyl-CpG binding domain protein 3) is a subunit of the NuRD, a multi-subunit complex containing nucleosome remodeling and histone deacetylase activities. Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as development and control of gene expression. It is generally associated with the repressive chromatin state. The complex series of events leading to this repressive state involve the coordinated regulation of DNA methyltransferases and two other groups of proteins called the Methyl-CpG binding proteins (MBD proteins) and the Kaiso family of proteins. The MBD family of proteins include MeCP2, MBD1, MBD2, MBD3 and MBD4. MBD3 regulates transcription by forming a Mi-2/NuRD complex with nucleosome remodeling and histone deacetylase (HDAC) activities in mammalian cells. MBD3 has been shown to interact not with 5-methylcytidine methylated DNA but with DNA containing 5-hydroxymethylcytosine (5-hmC) methylation. MDB3 co-localizes with TET1 and is required for normal levels of 5-hmC in embryonic stem cells.

Molecular Weight: 50 kDa

Gene ID: 53615

NCBI Accession: NP_001268382

Pathways: Chromatin Binding