RBPJ antibody

Catalog No. ABIN2668739

Cited in 1 publication. The Rat Monoclonal anti-RBPJ antibody (Clone 7A11) (ABIN2668739) specifically detects RBPJ in WB. The antibody is reactive with Human samples.

Quick Overview for RBPJ antibody (ABIN2668739)

Target: RBPJ (Recombination Signal Binding Protein For Immunoglobulin kappa J Region (RBPJ))

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This RBPJ antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 7A11

Product Details anti-RBPJ Antibody

Purpose: RBPJ antibody (mAb) (Clone 7A11)

Purification: Protein G Chromatography

Immunogen: This antibody was raised against full-length recombinant human RBPJ protein.

Isotype: IgG2b

Application Details

Application Notes: WB: 2 - 4 μg/mL dilution

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-RBPJ antibody (ABIN2668739)

Maier, Santak, Mantik, Grabusic, Kremmer, Hammerschmidt, Kempkes et al.: "A somatic knockout of CBF1 in a human B-cell line reveals that induction of CD21 and CCR7 by EBNA-2 is strictly CBF1 dependent and that downregulation of immunoglobulin M is partially CBF1 ..." in: Journal of virology, Vol. 79, Issue 14, pp. 8784-92, (2005) (PubMed).

Target Details for RBPJ

Target: RBPJ (Recombination Signal Binding Protein For Immunoglobulin kappa J Region (RBPJ))

Alternative Name: RBPJ

Background: RBPJ (Recombination Signal Binding Protein For Immunoglobulin Kappa J Region) is a transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations. Acts as a transcriptional repressor when it is not associated with Notch proteins. When associated with some NICD product of Notch proteins (Notch intracellular domain), it acts as a transcriptional activator that activates transcription of Notch target genes. Probably represses or activates transcription via the recruitment of chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins, respectively. Specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence. Binds specifically to methylated DNA.

Molecular Weight: 55 kDa

Gene ID: 3516

NCBI Accession: NP_005340

Pathways: Notch Signaling, Stem Cell Maintenance, Smooth Muscle Cell Migration