NF-kB p65 antibody (AA 526-539)

Catalog No. ABIN2668795

There is 1 publication for this product available. The Mouse Monoclonal anti-NF-kB p65 antibody is suitable to detect NF-kB p65 in samples from Human and Mouse. It has been validated for WB and ChIP.

Quick Overview for NF-kB p65 antibody (AA 526-539) (ABIN2668795)

Target: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This NF-kB p65 antibody is un-conjugated

Application: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP)

Clone: 112A1021

Product Details anti-NF-kB p65 Antibody

Binding Specificity: AA 526-539

Purpose: NFkB p65 antibody (mAb) (Clone 112A1021)

Purification: Affinity Purified

Immunogen: This NFkB p65 antibody was raised against a synthetic peptide corresponding to amino acid residues 526-539 of human NFkB p65.

Isotype: IgG1

Application Details

Application Notes: WB: 2 - 5 μg/mL dilution For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1 % Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required.

Restrictions: For Research Use only

Handling

Concentration: 0.5 μg/μL

Buffer: PBS containing 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: 4 °C,-20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Store at 4°C for short term. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-NF-kB p65 antibody (ABIN2668795)

Sun, Alkhoury, Wang, Foster, Radecke, Tam, Edwards, Facciotti, Armstrong, Knowlton, Newman, Passerini, Simon: "IRF-1 and miRNA126 modulate VCAM-1 expression in response to a high-fat meal." in: Circulation research, Vol. 111, Issue 8, pp. 1054-64, (2012) (PubMed).

Target Details for NF-kB p65

Target: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Alternative Name: NFkappaB p65

Background: NFkB p65 (Nuclear factor NF-kappa-B p65 subunit also known as NFkB p65 or RelA) is a member of the Rel family of transcription factors that are involved in processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. The Rel family includes c-Rel, RelA (NFkB p65, NFkB3) and RelB (I-Rel). Rel proteins form dimers with the NFkB p50 (proteolytically processed from p105/NFkB1) and p52 (proteolytically processed from p100/NFkB2) members of the NFkB family that bind DNA and activate transcription. The NFkB p65/p50 heterodimer is the most abundant form of NFkB. NFkB signaling is negatively regulated by the sequestration of the NFkB complex in the cytoplasm by its association with the IkB family of inhibitory proteins. The IkB Kinase (IKK) complex is the key enzyme involved in the activation and translocation of NFkB. The IKK complex is composed of two catalytic subunits (IKKa and IKKb) and a regulatory subunit (IKKg). Upon its activation, the IKK complex phosphorylates IkB proteins (IkBa, IkBa phospho Ser32, 36), which marks them for ubiquitination and degradation. This enables the NFkB complex to translocate to the nucleus where it regulates transcription by binding to DNA.

Molecular Weight: 65 kDa

Gene ID: 5970

NCBI Accession: NP_068810

Pathways: NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteins