CoREST antibody (C-Term)

Catalog No. ABIN2668804

The Rabbit Polyclonal anti-CoREST antibody is suitable to detect CoREST in samples from Human. It has been validated for WB.

Quick Overview for CoREST antibody (C-Term) (ABIN2668804)

Target: CoREST (RCOR1) (REST Corepressor 1 (RCOR1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CoREST antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CoREST Antibody

Binding Specificity: C-Term

Purpose: CoREST antibody (pAb)

Purification: Affinity Purified

Immunogen: This CoREST antibody was raised against a synthetic peptide derived from the C-terminal region of human CoREST.

Isotype: IgG

Application Details

Application Notes: WB*: 1:500 - 1:1,000 dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for CoREST

Target: CoREST (RCOR1) (REST Corepressor 1 (RCOR1))

Alternative Name: CoREST

Background: CoREST (REST corepressor 1, RCOR1) is a corepressor protein recruited by the REST, a neuronal gene-specific transcriptional repressor, to help repress neuron specific gene expression in non-neuronal cell lineages. CoREST is an essential component of the BHC repressor complex that also contains HDAC1, HDAC2, HMG20B/BRAF35, LSD1 (a lysine-4 specific histone demethylase) and PHF21A/BHC80. The Herpes Simplex virus ICP0 protein blocks the repression of viral genes by disrupting the interaction between CoREST and HDAC1/2.

Molecular Weight: 66 kDa

Gene ID: 23186

NCBI Accession: NP_055971

Pathways: Regulation of Hormone Metabolic Process, Chromatin Binding