MECP2 antibody

Catalog No. ABIN2668837

There is 1 publication for this product available. The Rat Monoclonal anti-MECP2 antibody is suitable to detect MECP2 in samples from Human, Mouse and Rat. It has been validated for WB, IHC, ICC, IP, IF and ChIP.

Quick Overview for MECP2 antibody (ABIN2668837)

Target: MECP2 (Methyl CpG Binding Protein 2 (MECP2))

Reactivity: Human, Mouse, Rat

Host: Rat

Clonality: Monoclonal

Conjugate: This MECP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP)

Clone: 4H7

Product Details anti-MECP2 Antibody

Purpose: MeCP2 antibody (mAb) (Clone 4H7)

Purification: Protein G Chromatography

Immunogen: This MeCP2 antibody was raised against a full-length protein corresponding to rat MeCP2.

Isotype: IgG2a

Application Details

Application Notes: The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications: ChIP IP ICC/IF IHC WB View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-MECP2 antibody (ABIN2668837)

Jost, Rottach, Milden, Bertulat, Becker, Wolf, Sandoval, Petazzi, Huertas, Esteller, Kremmer, Leonhardt, Cardoso: "Generation and characterization of rat and mouse monoclonal antibodies specific for MeCP2 and their use in X-inactivation studies." in: PLoS ONE, Vol. 6, Issue 11, pp. e26499, (2011) (PubMed).

Target Details for MECP2

Target: MECP2 (Methyl CpG Binding Protein 2 (MECP2))

Alternative Name: MeCP2

Background: Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as development and control of gene expression. It is generally associated with the repressive chromatin state. The complex series of events leading to this repressive state involve the coordinated regulation of DNA methyltransferases and two other groups of proteins called the Methyl-CpG binding proteins (MBD proteins) and the Kaiso family of proteins. The MBD family of proteins include MeCP2, MBD1, MBD2, MBD3 and MBD4. MeCP2 (methyl CpG binding protein 2, PPMX, MRX16, MRX79, MRXSL, AUTSX3, MRXS13) is a chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A.

Molecular Weight: 72 kDa

Gene ID: 29386

NCBI Accession: NP_073164

Pathways: Inositol Metabolic Process, Chromatin Binding, Synaptic Membrane