5-Hydroxymethylcytosine antibody

Catalog No. ABIN2668980

This Rabbit Polyclonal antibody specifically detects 5-Hydroxymethylcytosine in DB, MeDIP, IHC and IF. It has been mentioned in 15+ publications

Quick Overview for 5-Hydroxymethylcytosine antibody (ABIN2668980)

Target: 5-Hydroxymethylcytosine (5-hmC)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This 5-Hydroxymethylcytosine antibody is un-conjugated

Application: Dot Blot (DB), Methylated DNA Immunoprecipitation (MeDIP), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-5-Hydroxymethylcytosine Antibody

Purpose: 5-Hydroxymethylcytosine (5-hmC) antibody (pAb)

Purification: Protein A Chromatography

Immunogen: This 5-Hydroxymethylcytidine antibody was raised against 5-hydroxymethylcytidine conjugated to KLH and recognizes 5-hydroxymethylcytosine.

Isotype: IgG

Application Details

Application Notes: MeDIP: 1 - 2 μg per IP IHC (FFPE): 1 μg/mL dilution DB: 0.2 μg/mL dilution

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: PBS pH 7.5 containing 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-5-Hydroxymethylcytosine antibody (ABIN2668980)

Wiehle, Raddatz, Musch, Dawlaty, Jaenisch, Lyko, Breiling: "Tet1 and Tet2 Protect DNA Methylation Canyons against Hypermethylation." in: Molecular and cellular biology, Vol. 36, Issue 3, pp. 452-61, (2016) (PubMed).

Barazeghi, Gill, Sidhu, Norlén, Dina, Palazzo, Hellman, Stålberg, Westin: "5-Hydroxymethylcytosine discriminates between parathyroid adenoma and carcinoma." in: Clinical epigenetics, Vol. 8, pp. 31, (2016) (PubMed).

Uribe-Lewis, Stark, Carroll, Dunning, Bachman, Ito, Stojic, Halim, Vowler, Lynch, Delatte, de Bony, Colin, Defrance, Krueger, Silva, Ten Hoopen, Ibrahim, Fuks, Murrell: "5-hydroxymethylcytosine marks promoters in colon that resist DNA hypermethylation in cancer." in: Genome biology, Vol. 16, pp. 69, (2015) (PubMed).

Chen, Aksoy, Gonnot, Osteil, Aubry, Hamela, Rognard, Hochard, Voisin, Fontaine, Mure, Afanassieff, Cleroux, Guibert, Chen, Vallot, Acloque, Genthon, Donnadieu, De Vos, Sanlaville, Guérin, Weber et al.: "Reinforcement of STAT3 activity reprogrammes human embryonic stem cells to naive-like pluripotency. ..." in: Nature communications, Vol. 6, pp. 7095, (2015) (PubMed).

Eleftheriou, Pascual, Wheldon, Perry, Abakir, Arora, Johnson, Auer, Ellis, Madhusudan, Ruzov: "5-Carboxylcytosine levels are elevated in human breast cancers and gliomas." in: Clinical epigenetics, Vol. 7, pp. 88, (2015) (PubMed).

Zhao, Yang, Wang, Chong, Yin, Song, Zhao, Li, Huang, Sun, Wu, Jin, Song, Zhu, Jiang, Rendtlew Danielsen, Xu, Yang, Wang: "Redox-active quinones induces genome-wide DNA methylation changes by an iron-mediated and Tet-dependent mechanism." in: Nucleic acids research, Vol. 42, Issue 3, pp. 1593-605, (2014) (PubMed).

Müller, Bauer, Siegl, Rottach, Leonhardt: "TET-mediated oxidation of methylcytosine causes TDG or NEIL glycosylase dependent gene reactivation." in: Nucleic acids research, Vol. 42, Issue 13, pp. 8592-604, (2014) (PubMed).

Hashimoto, Pais, Zhang, Saleh, Fu, Dai, Corrêa, Zheng, Cheng: "Structure of a Naegleria Tet-like dioxygenase in complex with 5-methylcytosine DNA." in: Nature, Vol. 506, Issue 7488, pp. 391-5, (2014) (PubMed).

Colquitt, Allen, Barnea, Lomvardas: "Alteration of genic 5-hydroxymethylcytosine patterning in olfactory neurons correlates with changes in gene expression and cell identity." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 36, pp. 14682-7, (2013) (PubMed).

Yamaguchi, Hong, Liu, Inoue, Shen, Zhang, Zhang: "Dynamics of 5-methylcytosine and 5-hydroxymethylcytosine during germ cell reprogramming." in: Cell research, Vol. 23, Issue 3, pp. 329-39, (2013) (PubMed).

Shen, Wu, Diep, Yamaguchi, DAlessio, Fung, Zhang, Zhang: "Genome-wide analysis reveals TET- and TDG-dependent 5-methylcytosine oxidation dynamics." in: Cell, Vol. 153, Issue 3, pp. 692-706, (2013) (PubMed).

Leitch, McEwen, Turp, Encheva, Carroll, Grabole, Mansfield, Nashun, Knezovich, Smith, Surani, Hajkova: "Naive pluripotency is associated with global DNA hypomethylation." in: Nature structural & molecular biology, Vol. 20, Issue 3, pp. 311-6, (2013) (PubMed).

Bocker, Tuorto, Raddatz, Musch, Yang, Xu, Lyko, Breiling: "Hydroxylation of 5-methylcytosine by TET2 maintains the active state of the mammalian HOXA cluster." in: Nature communications, Vol. 3, pp. 818, (2012) (PubMed).

Wu, DAlessio, Ito, Wang, Cui, Zhao, Sun, Zhang: "Genome-wide analysis of 5-hydroxymethylcytosine distribution reveals its dual function in transcriptional regulation in mouse embryonic stem cells." in: Genes & development, Vol. 25, Issue 7, pp. 679-84, (2011) (PubMed).

Li, Cai, Cai, Wang, Zhang, Petersen, Yang, Xu: "Deletion of Tet2 in mice leads to dysregulated hematopoietic stem cells and subsequent development of myeloid malignancies." in: Blood, Vol. 118, Issue 17, pp. 4509-18, (2011) (PubMed).

Target Details for 5-Hydroxymethylcytosine

Target: 5-Hydroxymethylcytosine (5-hmC)

Alternative Name: 5-Hydroxymethylcytosine

Target Type: Chemical

Background: Active Motif offers two polyclonal antibodies that recognize 5-hydroxymethylcytosine, a whole serum version (39769) and a purified IgG version (39791).Both are validated for use in methyl DNA immunoprecipitation (MeDIP). For customers that require the ability to quantitate the amount of IgG in the MeDIP reaction, the purified IgG version (39791) is recommended. The whole serum version (39769) is very high titre and should be used carefully (0.1 - 0.5 µL per IP) as not to generate high non-specific background. The whole serum version (39769) has been used successfully in immunofluorescence (IF, Ito et al, 2010), and the purified IgG version (39791) is likely to work in this application as well. DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease. 5-methylcytosine is a modified base that is found in the DNA of plants and vertebrates. A second type of DNA methylation exists, 5-hydroxymethylcytosine (5-hydroxy methylcytosine, 5-hmC). This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of cytosine oxygenases. This antibody was developed specifically to distinguish 5-hydroxymethylcytosine from 5-methylcytosine as conventional methods (enrichment by antibody or methyl DNA binding protein, enzymatic digestion and bisulfite sequencing) cannot do so. It is possible that 5-hydroxymethylcytosine (5-hmC) represents a pathway to demethylate DNA, as 5-hydroxymethylcytosine is repaired as mismatched DNA and replaced with unmethylated cytosine.