ARF1 antibody

Catalog No. ABIN2688655

There are 2+ publications for this product available. The Rabbit Polyclonal anti-ARF1 antibody is suitable to detect ARF1 in samples from Potato, Physcomytrium Patens, Petunia hybrida, Oryza sativa, Nicotiana tabacum, Lilium longiflorum (Trumpet lily), Elaeis, Chlamydomonas reinhardtii, Arabidopsis thaliana, Alfalfa and Actinidia chinensis. It has been validated for WB, IF and EM.

Quick Overview for ARF1 antibody (ABIN2688655)

Target: ARF1 (ADP-Ribosylation Factor 1 (ARF1))

Reactivity: Potato, Physcomytrium Patens, Petunia hybrida, Oryza sativa, Nicotiana tabacum, Lilium longiflorum (Trumpet lily), Elaeis, Chlamydomonas reinhardtii, Arabidopsis thaliana, Alfalfa, Actinidia chinensis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Electron Microscopy (EM)

Product Details anti-ARF1 Antibody

Purpose: Anti-ARF1 | ADP-ribosylation factor 1

Cross-Reactivity (Details): Actinidia chinensis, Arabidopsis thaliana, Chlamydomonas reinhardtii, Elaeis sp. , Lilium longiflorum, Medicago truncatula, Nicotiana tabacum, Oryza sativa, Petunia hybrida cv Mitchell, Physcomitrium patens, Solanum tuberosum
Not reactive in: Centella asiatica, Microsporidia sp.

Predicted Reactivity: Brassica juncea,Brassica napus,Capsella rubella,Capsicum annum,Cucumis sp.,Daucus carota,Elaeis guineensis,Glycine max,Helleborus orientalis,Hordeum vulgare,Medicago truncatula,Nannochloropsis gaditana,Nicotiana benthamina,Ostreococcus tauri,Populus trichocarpa,Triticum aestivum,Zea mays

Purification: Serum

Immunogen: Recombinant GST fusion of full length of Arabidopsis thaliana ARF1 (P36397

Application Details

Application Notes: 1 : 1000 (IF), 1 : 100 (IG), 1 : 1000 (WB)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Serum

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

References for anti-ARF1 antibody (ABIN2688655)

Wang, Ito, Uehara, Naito, Takano: "UDP-D-galactose synthesis by UDP-glucose 4-epimerase 4 is required for organization of the trans-Golgi network/early endosome in Arabidopsis thaliana root epidermal cells." in: Journal of plant research, (2015) (PubMed).

Pertl, Schulze, Obermeyer: "The pollen organelle membrane proteome reveals highly spatial-temporal dynamics during germination and tube growth of lily pollen." in: Journal of proteome research, Vol. 8, Issue 11, pp. 5142-52, (2009) (PubMed).

Target Details for ARF1

Target: ARF1 (ADP-Ribosylation Factor 1 (ARF1))

Alternative Name: Arf1

Background:

Background: The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. It is a small GTPase that undergoes a GDP/GTP nucleotide exchange cycle and it is an important regulator of cellular trafficking.

Additional information: Cellular [compartment marker] of Golgi in immunolocalization and COP1 in western blot

Molecular Weight: 21 kDa (Arabidopsis thaliana)

Gene ID: 819330

UniProt: P36397

Pathways: Transition Metal Ion Homeostasis, Inositol Metabolic Process