ARF1 antibody

Catalog No. ABIN2688655

The Rabbit Polyclonal anti-ARF1 antibody has been validated for WB and IF. It is suitable to detect ARF1 in samples from Oryza sativa, Nicotiana tabacum, Lilium longiflorum (Trumpet lily), Chlamydomonas reinhardtii and Arabidopsis thaliana. There are 2+ publications available.

Quick Overview for ARF1 antibody (ABIN2688655)

Target: ARF1 (ADP-Ribosylation Factor 1 (ARF1))

Reactivity: Oryza sativa, Nicotiana tabacum, Lilium longiflorum (Trumpet lily), Chlamydomonas reinhardtii, Arabidopsis thaliana

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-ARF1 Antibody

Cross-Reactivity (Details): No cross-reactivity with: Microsporidia sp.

Characteristics: Expected / apparent Molecular Weight of the Antigene: 21 kDa (Arabidopsis thaliana)

Purification: serum

Immunogen: recombinant GST fusion of full length of Arabidopsis thaliana ARF1 (P36397, At2g47170)

Application Details

Application Notes: Recommended Dilution: 1 : 1000 with standard ECL (WB), 1 : 1000 (IF).
Cellular [compartment marker] of Golgi in immunolocalization and COP1 in western blot

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 200 µL of sterile water.

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Storage: -20 °C

References for anti-ARF1 antibody (ABIN2688655)

Wang, Ito, Uehara, Naito, Takano: "UDP-D-galactose synthesis by UDP-glucose 4-epimerase 4 is required for organization of the trans-Golgi network/early endosome in Arabidopsis thaliana root epidermal cells." in: Journal of plant research, (2015) (PubMed).

Pertl, Schulze, Obermeyer: "The pollen organelle membrane proteome reveals highly spatial-temporal dynamics during germination and tube growth of lily pollen." in: Journal of proteome research, Vol. 8, Issue 11, pp. 5142-52, (2009) (PubMed).

Target Details for ARF1

Target: ARF1 (ADP-Ribosylation Factor 1 (ARF1))

Alternative Name: Arf1

Background: The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. It is a small GTPase that undergoes a GDP/GTP nucleotide exchange cycle and it is an important regulator of cellular trafficking.

Molecular Weight: 21 kDa (Arabidopsis thaliana)

Pathways: Transition Metal Ion Homeostasis, Inositol Metabolic Process