IL2RG antibody (Interleukin 2 Receptor, gamma) (Biotin)

Details for Product anti-IL2RG Antibody No. ABIN2688956
Antigen
  • Ab2-183
  • Cd132
  • CD132
  • CIDX
  • IL-2RG
  • IMD4
  • P64
  • SCIDX
  • SCIDX1
  • [g]c
  • gamma(c)
  • gc
  • interleukin 2 receptor subunit gamma
  • interleukin 2 receptor subunit gamma L homeolog
  • interleukin 2 receptor, gamma chain
  • IL2RG
  • il2rg.L
  • Il2rg
Reactivity
Mouse (Murine)
109
67
38
2
1
Host
Rat
105
14
11
Clonality (Clone)
Monoclonal ()
Conjugate
This IL2RG antibody is conjugated to Biotin
10
10
6
5
4
2
2
2
2
2
2
2
1
1
1
1
1
Application
Blocking Reagent (BR), Flow Cytometry (FACS), Neutralization (Neut)
69
48
28
18
13
6
4
4
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2
1
1
1
Options
Brand BD Pharmingen™
Clone TUGm2
Isotype IgG2b, kappa
Characteristics The TUGm2 antibody reacts with Mouse CD132 which is also known as the common γ subunit (γc). The γc chain subunit is shared by the IL-2, IL-4, IL-7, IL-9, and IL-15 receptor complexes. The γc receptor is expressed constitutively at low levels by most lymphocytes, myeloid cells, embryonic thymocytes, and lymphoid cell lines. The γc receptor is a 75-80 kD transmembrane glycoprotein which mediates signal transducing activities of cytokine receptor complexes with which it is associated. TUGm2 antibody can significantly block the binding of and proliferative response to IL-4 by CTLL-2 cells. It can also inhibit binding to high-affinity IL-2 receptors expressed by CTLL-2 cells. By itself, the TUGm2 antibody reportedly fails to significantly inhibit CTLL-2 proliferative responses to IL-2. However, when added along with TM-β1 (Cat. No. 553359), specific for the mouse IL-2Rβ chain, TUGm2 was shown to synergistically reduce IL-2-driven CTLL-2 proliferative responses. TUGm2 can reduce the IL-7-induced proliferation of Con A-activated mouse splenocytes and the IL-7 responsive pre-B cell line, IxN/2b. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.

BD Pharmingen™ Biotin Rat Anti-Mouse CD132 - Biotin - Clone TUGm2 - Isotype Rat IgG2b, κ - Reactivity Ms - 0.5 mg
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Plasmids, Primers & others Plasmids, Primers & others IL2RG products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name CD132 (IL2RG Antibody Abstract)
Background Synonyms: Cytokine Common γ chain, γc
Pathways JAK-STAT Signaling, Growth Factor Binding
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
Storage 4 °C
Storage Comment Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Product cited in: Kondo, Takeshita, Higuchi, Nakamura, Sudo, Nishikawa, Sugamura: "Functional participation of the IL-2 receptor gamma chain in IL-7 receptor complexes." in: Science (New York, N.Y.), Vol. 263, Issue 5152, pp. 1453-4, 1994 (PubMed).

Kondo, Takeshita, Ishii, Nakamura, Watanabe, Arai, Sugamura: "Sharing of the interleukin-2 (IL-2) receptor gamma chain between receptors for IL-2 and IL-4." in: Science (New York, N.Y.), Vol. 262, Issue 5141, pp. 1874-7, 1994 (PubMed).

Guesdon, Ternynck, Avrameas: "The use of avidin-biotin interaction in immunoenzymatic techniques." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 27, Issue 8, pp. 1131-9, 1979 (PubMed).

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